Hepatotoxic effect of popular energy drink: biochemical and histopathological evaluation.

Document Type : Original articles


1 Forensic medicine and clinical toxicology department, Faculty of Medicine, Cairo University, Egypt,

2 Forensic medicine and clinical toxicology department, Faculty of Medicine, Beni-Suef University, Egypt,

3 Pathology department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.


Objective: To assess biochemical and histopathological hepatotoxic effect of popular energy drink (POWER HORSE) Design: randomized controlled trial study. Materials and Methods: 40 adult albino rats were randomly distributed into two groups, each group formed of twenty rats, group (I), received tap water, group (II), received POWER HORSE, 3.8ml/100g orally, every day for continuous twenty one (21) days. All animals were scarified. Blood samples were gained for biochemical analysis. Histopathological examination of liver was performed through Hematoxylin and Eosin staining. Results: POWER HORSE energy drink treated group showed significant increase in AST, ALT and ALP levels when compared to the CONTROL group. Group received POWER HORSE energy drink showed detrimental effects of on liver histopathological examination with severe degenerative changes in liver tissue.  Conclusions: POWER HORSE energy drink showed high degree of detrimental effects on both liver functions and histopathological examination of liver tissue revealing the hazardous effect og energy drinks on health.


Main Subjects

  1. Higgins, J. P.; Babu, K.; Deuster, P. A. and Shearer, J. (2018). Energy drinks: A contemporary issues paper. Current sports medicine reports, 17(2), 65-72.
  2. Valle, M. C.; Couto-Pereira, N. S.; Lampert, C.; Arcego, D. M.; Toniazzo, A. P.; Limberger, R. P.; Dallegrave, E.; Dalmaz, C.; Arbo, M.D. and Leal, M. B. (2018): Energy drinks and their component modulate attention, memory, and antioxidant defenses in rats. European journal of nutrition, 57(7), 2501-2511.
  3. Kamar, S. A.; Malak, H. W. A. and Saad, S. A. (2020). Effect of caffeinated energy drinks on the structure of hippocampal cornu ammonis 1 and dentate gyrus of adult male albino rats. Anatomy & Cell Biology, 53(3), 330-341.
  4. Curran, C. P. and Marczinski, C. A. (2017). Taurine, caffeine, and energy drinks: Reviewing the risks to the adolescent brain. Birth defects research, 109(20), 1640-1648.
  5. Heckman, M. A.; Sherry, K. and De Mejia, E. G. (2010). Energy drinks: an assessment of their market size, consumer demographics, ingredient profile, functionality, and regulations in the United States. Comprehensive Reviews in food science and food safety, 9(3), 303-317
  6. Leal, W. E. and Jackson, D. B. (2018). Energy drinks and escalation in drug use severity: An emergent hazard to adolescent health. Preventive medicine, 111, 391-396.
  7. Gubina-Vakulyck, G.; Gorbach, T.; Onishchenko, A.; Novikova, V.; Nakonechna, O.; Tryfonova, N. and et al. (2019). Apoptosis and regeneration of hepatocytes in rats orally exposed to caffeinated energy drinks. Comparative Clinical Pathology, 1-7.
  8. Almehmadi, A. B. M. (2017). Effect of “Red Bull” energy drink on some neurotransmitters content and histological structure of cerebral cortex in male albino rats. Life Science Journal, 14(1).
  9. Reitman, S. and Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American journal of clinical pathology28(1), 56-63.
  10. Ng, V. (2002). Effects of Disease on Clinical Laboratory Tests, Vol. 1 and 2. DS Young and RB Friedman, eds. Washington, DC: AACC Press, 2001, $199.00 ($159.00 AACC members), softcover. ISBN 1-890883-45-X. Clinical Chemistry48(4), 682-683.
  11. Bancroft, D.;Stevens, A. and Turner, R. (1996): The haematoxylin and eosin. Theory and practice of histological techniques. 4th ed, Ch 6, pp.99–112.
  12. Akande, I. S. and Banjoko, O. A. (2011). Assessment of biochemical effect of “Power Horse” energy drink on hepatic, renal and histological functions in Sprague Dawley rats.
  13. Mansy, W.; Alogaiel, D. M.; Hanafi, M. and Zakaria, E. (2017). Effects of chronic consumption of energy drinks on liver and kidney of experimental rats. Tropical Journal of Pharmaceutical Research, 16(12), 2849-2856.
  14. Ugwuja, E. (2014). Biochemical effects of energy drinks alone or in combination with alcohol in normal albino rats. Advanced pharmaceutical bulletin, 4(1), 69.
  15. Vivekanandarajah, A., Ni, S. and Waked, A. (2011). Acute hepatitis in a woman following excessive ingestion of an energy drink: a case report. Journal of medical case reports, 5(1), 1-3.
  16. Eltahir, H. M.; Alamri, G.; Alamri, A.; Aloufi, A.; Nazmy, M.; Elbadawy, H. and Abouzied, M. (2020). The metabolic disorders associated with chronic consumption of soft and energy drinks in rats. Acta Biochimica Polonica, 67(1), 79-84.