The possible protective effect of pumpkin seed oil on dutasteride-induced changes on spleen of adult male albino rats: Histological and immunohistochemical study

Document Type : Original articles

Authors

1 Professor of Histology and Cell Biology Faculty of Medicine, Tanta University

2 Anatomy and Embryology department, Faculty of Medicine, Tanta University, Egypt..

3 Histology &cell biology Department _Faculty of Medicine _Tanta University

Abstract

Background: Dutasteride is an anti-androgen used in the treatment of prostatic diseases. Pumpkin seed oil (PSO) is a functional food oil with an antioxidant and immunomodulatory actions. Aim: assessing the possible protective effect of PSO on dutasteride-induced changes on spleen of adult male albino rats via histological and immunohistochemical methods. Methods: 30 male albino rats 200-250 gm divided into; Control group, Dutasteride low dose (DLD): rats received dutasteride 0.05 ml /day, Dutasteride high dose (DHD): rats received dutasteride 0.15 ml/ day, DLD + PSO: dutasteride low dose group treated with 1.5 ml/kg/day of PSO, DHD + PSO: dutasteride high dose treated with 1.5 ml/kg/day of PSO. Doses were orally for 42 days. Results: DLD &DHD groups (Groups II &III) showed marked histopathological changes dose dependently. There was dilated blood sinusoids in the subcapsular areas & throughout the parenchyma, disorganization, dilatation& expansion of the red pulp with reduced splenic cords, disorganization & reduction in the size of the white pulp lymphoid follicles having lymphocytes with pyknotic nuclei and vacuolated cytoplasm, macrophages containing apoptotic bodies, dilated central arteriole with detached endothelium and vacuolated wall, and significantly increased collagen mean area%. Also, significantly increased mean number of immune-positive cells for CD68& TGF-β while decreased for CD3 immunohistochemical expression. Regarding the DLD+PSO& DHD+PSO groups (Groups III& IV); marked amelioration of the previously mentioned results was observed. Conclusion: PSO has an ameliorative effect on the histopathological changes on rat spleen induced by dutasteride. So, PSO is beneficial in the treatment of side effects of dutasteride.

Keywords

Main Subjects

References

  1. Higazy A, Shorbagy A, Shabayek M, Radwan A, Halim GN, Osman T: Short course of dutasteride in treatment of a refractory category IIIB chronic prostatitis (A placebo-controlled study). Prostate International. 2022;10(4):213-217.
  2. Sugie S, Mukai S, Tsukino H, Iwamoto H, Kobayashi T,Toda Y, Kamoto T: Effect of dutasteride on microvessel density in benign prostatic hyperplasia. In Vivo. 2014;28(3):355-359.
  3. Mohamad BJ, Obaid HH, Zghair FA: Study the toxic effect of different doses of duprost on spleen of albino mice. Health Sciences. 2019;8(3): 85-89.
  4. Uroko RI, Aaron CF, Uche ME, Aguwamba C, Ogwo EU, Nweje-Anyalowu PC, Ijioma SN: Effect of Aju Mbaise on sperm morphology, semen quality, sex hormonal levels, gonadosomatic index and testicular histology of Avodart-induced rats. Plant Biotechnology Persa. 2022; 4(2):22-36.
  5. Yilmaz-Oral D, Onder A, Kaya-Sezginer E, Oztekin CV, Zor M, Gur S: Restorative effects of red onion (Allium cepa L.) juice on erectile function after-treatment with 5α-reductase inhibitor in rats. International Journal of Impotence Research. 2022;34(3):269-276.
  6. Aghaei S, Nikzad H, Taghizadeh M, Tameh AA, Taherian A, Moravveji A: Protective effect of Pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with Cyclophosphamide. Andrologia. 2014; 46(8):927-935.
  7. Bora NS, Pathak MP, Mandal S, Mazumder B, Policegoudra R, Raju PS, Chattopadhyay P: Safety assessment and toxicological profiling of a novel combinational sunprotective dermal formulation containing melatonin and pumpkin seed oil. Regulatory Toxicology and Pharmacology. 2017;(89):1-12.
  8. Zaki NG, Shabana FM, El-sherif NM, El-Habeby MM: The protective effect of pumpkin seed oil on L-thyroxine-induced rat testicular oxidative stress. Menoufia Medical Journal. 2022;35(2):351-358.
  9. Niazi MK, Hassan FH, Ashfaq J, Ejaz F, Aamir Z, Imran S: the Nutritional and Potential Health Effect of Pumpkin seeds: Health Effect of Pumpkin seeds. Pakistan BioMedical Journal. 2022;17-21.
  10. Eraslan G, Kanbur M, Aslan Ö, Karabacak M: The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice. Environmental toxicology. 2013;28(12):681-688.
  11. Rouag M, Berrouague S, Djaber N, Khaldi T, Boumendjel M, Taibi F, Abdennour C, Boumendjel A, Messarah M: Pumpkin seed oil alleviates oxidative stress and liver damage induced by sodium nitrate in adult rats: biochemical and histological approach. African Health Sciences. 2020;20(1):413-425.
  12. Lyttle KA, Gossell‐Williams MD, Clarke T, Gardner M, Simon O, Influence of Pumpkin Seed Oil Supplementation on Cardiovascular and Histological Outcomes in Female Non‐ovariectomized and Ovariectomized Rats. 2008;719-729.
  13. Dyson MC, Jirkof P, Lofgren J, Nunamaker EA, Pang D: Anesthesia and analgesia in laboratory animals. 3rd edition. American College of Laboratory Animal Medicine Series. Elsevier Inc. Academic Press. UK; 2023.
  14. Suvarna KS, Layton C, Bancroft JD. “Bancroft’s Theory and Practice of Histological Techniques”. Chapter 11th “Connective and other mesenchymal tissues with their stains”, “Suvarna KS, Layton C, Bancroft JD”. Elsevier health sciences; 2018.
  15. Youssef S, Salah M: Differential expression of CD3, TNF-α, and VEGF induced by olanzapine on the spleen of adult male albino rats and the possible protective role of vitamin C. Biomedicines. 2019;7(2): 39-49.
  16. Bindaputri JF, Sudiono J: CD68 Expression on Macrophages as Anti-Inflammatory Effect of Tamarillo (Solanum betaceum Cav.) Fruit Peel Ethanol Extract (Study on Carrageenan-Induced Buccal Mucosa of Rats). Jurnal Biomedika dan Kesehatan. 2024;7(1):6-16.
  17. El-Shafei R, El-Adl M, Ali H, Nomier Y: Ameliorative effect of Arabic gum Acacia and mori extracts in streptozotocin-induced diabetic rats: implications of Cas-3 and TGF-β. European Review for Medical & Pharmacological Sciences. 2023;27(7):2845-2857.
  18. Anastassakis K., Dutasteride, in Androgenetic Alopecia From A to Z. Vol. 2 Drugs, Herbs, Nutrition and Supplements. Springer; 2022.
  19. Daryanto B, Suharibawa IGLA, Purnomo AF, Nurhadi P, Wardhani V: The Effect Of Continuously Dutasteride Monotherapy on The Expression Of Protein Kinase C-Alpha Enzyme In BPH Model Of Wistar Strain Rattus Novergicus Rat. Medical Archives. 2023;77(3): 202-212.
  20. Daryanto B, Naim HY, Budaya TN: The Effect of Tamsulosin, Dutasteride Monotherapy and Tamsulosin-Dutasteride Combination on Prostate Smooth Muscle Contractility in BPH Model Wistar Strain Rattus Novergicus. Medical Archives. 2023;77(1):13-23.
  21. Aslam BF, Alam SS, Piracha MI, Zulfiqar T, Jabbar S, Mansoor M: Prophylactic effect of citrullus lanatus & curcurbita pepo seeds extract versus dutasteride on hormonal parameters in benign prostatic hyperplasia rats. Journal of Akhtar Saeed Medical & Dental College. 2023;5(3):157-163.
  22. Grootveld AK, Kyaw W, Panova V, Lau AW, Ashwin E, Seuzaret G, Dhenni R, Bhattacharyya ND, Khoo WH, Biro M: Apoptotic cell fragments locally activate tingible body macrophages in the germinal center. Cell. 2023;186(6):1144-1161.
  23. Gotur SP, Wadhwan V: Tingible body macrophages. Journal of Oral and Maxillofacial Pathology. 2020;24(3):418-420.
  24. von Kleeck R, Castagnino P, Roberts E, Talwar S, Ferrari G, Assoian RK: Decreased vascular smooth muscle contractility in Hutchinson–Gilford progeria syndrome linked to defective smooth muscle myosin heavy chain expression. Scientific reports. 2021;11(1):10625.
  25. Mizoguchi S, Mori K, Shin T, Wang Z, DeFranco DB, Yoshimura N, Mimata H: Effects of dutasteride in a rat model of chemically induced prostatic inflammation. Potential role of estrogen receptor β. The Prostate. 2020:80(16) 1413-1420.
  26. Uroko RI, Ogwo EU, Nweje-Anyalowu P, Obiwuru I, Aaron CF, Mba OJ: Ameliorative potentials of Aju Mbaise extract (AME) on Dutasteride induced oxidative stress and hepatic injury in rats. Journal of Medicinal Herbs. 2023;13(4):17-26.
  27. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A: Oxidative stress: harms and benefits for human health. Oxidative medicine and cellular longevity. 2017; 2017.
  28. Monageng E, Offor U, Takalani NB, Mohlala K, Opuwari CS: A review on the impact of oxidative stress and medicinal plants on Leydig cells. Antioxidants. 2023;12(8):1559-1556.
  29. Abdelazim AM, Abomughaid MM: Oxidative stress: an overview of past research and future insights. All Life. 2024;17(1):2316092-2316112.
  30. Obeagu EI, Igwe MC, Obeagu GU: Oxidative stress’s impact on red blood cells: Unveiling implications for health and disease. Medicine. 2024:103(9):e37360-e37370.
  31. Fujiyama S, Nakahashi-Oda C, Abe F, Wang Y, Sato K, Shibuya A: Identification and isolation of splenic tissue-resident macrophage sub-populations by flow cytometry. International Immunology. 2019; 31(1): 51-56.
  32. Türkmen N, Eren B, Fedakar R, Akgöz S: The significance of hemosiderin deposition in the lungs and organs of the mononucleated macrophage resorption system in infants and children. Journal of Korean medical science. 2008:23(6):1020-1030.
  33. Massaccesi L, Galliera E, Romanelli MMC: Erythrocytes as markers of oxidative stress related pathologies. Mechanisms of Ageing and Development. 2020;191:111333-111343.
  34. Suttie AW: Histopathology of the spleen. Toxicologic pathology. 2006; 34(5):466-503.
  35. Kim HK, Zhao C, Choi BR, Chae HJ, Kim DS, Park JK: Is transforming growth factor-β signaling activated in human hypertrophied prostate treated by 5-alpha reductase inhibitor? Disease markers. 2013;(35):679-685.
  36. Gamat M, McNeel DG: Androgen deprivation and immunotherapy for the treatment of prostate cancer. Endocrine-related cancer. 2017;24(12):T297-T310.
  37. Jahan F, Islam MB, Moulick SP, Al Bashera M, Hasan MS, Tasnim N, Saha T, Boby F, Waliullah M, Saha AK: Nutritional characterization and antioxidant properties of various edible portions of Cucurbita maxima: A potential source of nutraceuticals. Heliyon. 2023;9(6):1-7
  38. Ceclu L, Mocanu D, GNistor OV: Pumpkin–health benefits. Journal of agroalimentary processes and technologies. 2020;26(3): 241-246.
  39. Hu T, Zhou L, Kong F, Wang S, Hong K, Lei F, He D: Effects of Extraction Strategies on Yield, Physicochemical and Antioxidant Properties of Pumpkin Seed Oil. Foods. 2023;12(18):351-361.
  40. Zhang G, Li ZFu M: Comparison of quality and oxidative stability of pumpkin seed oil (Cucurbita maxima) between conventional and enzymatic extraction methods. Sustainable Food Technology. 2024;1-7.
  41. Abdelnour SA, Metwally MG, Bahgat LB, Naiel MA: Pumpkin seed oil–supplemented diets promoted the growth productivity, antioxidative capacity, and immune response in heat-stressed growing rabbits. Tropical Animal Health and Production. 2023;55(1):55-65.
  42. Singh A, Kumar V: Phyto-chemical and bioactive compounds of pumpkin seed oil as affected by different extraction methods. Food Chemistry Advances. 2023;(2):100211-100221.
  43. Amin MZ, Rity TI, Uddin MR, Rahman MM, Uddin MJ: A comparative assessment of anti-inflammatory, anti-oxidant and anti-bacterial activities of hybrid and indigenous varieties of pumpkin (Cucurbita maxima Linn.) seed oil. Biocatalysis and agricultural biotechnology. 2020; (28)101767-101777.
  44. Singh A, Kumar V: Pumpkin seeds as nutraceutical and functional food ingredient for future: A review. Grain & Oil Science and Technology. 2024;12-29.
  45. Singh B, Singh JP, Kaur A, Singh N: Phenolic composition and antioxidant potential of grain legume seeds: A review. Food research international. 2017;(101):1-16.
  46. El-mehi AE: Effect of pumpkin seed oil on experimentally induced early liver fibrosis in the adult male albino rat. Egyptian Journal of Histology. 2023;46(2):796-809.
  47. Naghii MR, Mofid M: Impact of daily consumption of iron fortified ready-to-eat cereal and pumpkin seed kernels (Cucurbita pepo) on serum iron in adult women. Biofactors. 2007;30(1):19-26.

 

Files

Share

How to cite

Statistics