Effect of Adipose Tissue-Derived Stem Cells on Cyclophosphamide-Induced Premature Ovarian Failure in Adult Albino Rat: Structural and Immunohistochemical Study

Document Type : Original articles

Authors

1 Anatomy and Embryology department, Faculty of Medicine, Beni-Suef University

2 Histology department, Faculty of Medicine, Beni-Suef University

3 Anatomy and Embryology department, Faculty of Medicine, Cairo University

Abstract

This study aimed to detect the therapeutic effect of ADSCs on cyclophosphamide-induced POF in rats. forty female albino rats 2 months old (150-200 Kg) have randomly divided into four equal groups, group I (control group): received no medications, group II (cyclophosphamide- treated group): rats received an intra-venous injection (IV) of a single dose of cyclophosphamide (200 mg/kg/ cycle) for two cycles and two weeks after the administration of the cyclophosphamide, they will be sacrificed, group III (cyclophosphamide (200 mg/kg/ cycle) +Stem cell treated group). After 1 week of cyclophosphamide, the animals received 2 million units of ADSCs/ animal I.V. group IV(recovery group) ; the recovery group chemotherapy-treated with cyclophosphamide for two cycles and after 21 days, animals were sacrificed. The Ovarian specimens were examined using Hematoxylin and Eosin, Masson's trichrome, and immunohistochemical (Proliferating Cell Nuclear Antigen broth, B- Cell Lymphoma-2) antibodies. Blood hormone levels (Follicular Stimulating Hormone, Luteinizing Hormone, and Estradiol-2) were measured in all groups. Examination of ovarian sections revealed normal architecture in group I. pathological changes accompanied cyclophosphamide in groups II and IV. Reversal of these changes with the restoration of normal architecture was detected in group III. ADSCs, restored ovarian function and reversed follicular depletion induced by cyclophosphamide. ADSCs, increased expression of PCNA and Bcl2 and lowered collagen fibers expression, reversing the harmful effects of cyclophosphamide. In Conclusion: ADSCs ameliorated cyclophosphamide-induced premature ovarian failure in rats. So, it might be a promising strategy in fighting chemotherapy induced-premature ovarian failure.

Keywords

Main Subjects

References

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